The G Protein Coupled Receptor -
Oligomerization Knowledge Base Project

If checked, GPCR-OKB will search for Oligomers.

Please specify the name of the oligomer of interest made up of the names of the protomers that form its constituent parts. The "Oligomer" option is checked by default.

Example: If you would like to retrieve information about an oligomer of the dopamine D2 receptor, you can either write "dopamine" or "D2".

If you select the "With Phenotypic Changes" option, you will limit your search to only oligomers with demonstrated phenotypic changes.

If you select the "With Proposed Interfaces" option, you can retrieve available information about predicted interfaces of dimerization/oligomerization.

If you select the "With In Vivo Evidence" option, you will limit your search to Oligomers for which at least one of the following 3 criteria are satisfied: 1) Evidence for physical association in native tissue or primary cells; 2) Identification of specific functional property in native tissue; 3) Use of knockout animals or RNAi technology. These criteria are NC-IUPHAR recommendations to recognize a bona fide functional GPCR oligomer, and were reported in detail in Pin J-P, et al., International Union of Basic and Clinical Pharmacology. LXVII. Recommendations for the Recognition and Nomenclature of G Protein-Coupled Receptor Heteromultimers. Pharmacological Reviews 2007, 59(1):1-9.

If you select the "Evidence for Physiological Relevance" option, you will limit your search to Oligomers for which at least two of the following 3 criteria are satisfied: 1) Evidence for physical association in native tissue or primary cells; 2) Identification of specific functional property in native tissue; 3) Use of knockout animals or RNAi technology. These criteria are NC-IUPHAR recommendations to recognize a bona fide functional GPCR oligomer, and were reported in detail in Pin J-P, et al., International Union of Basic and Clinical Pharmacology. LXVII. Recommendations for the Recognition and Nomenclature of G Protein-Coupled Receptor Heteromultimers. Pharmacological Reviews 2007, 59(1):1-9.

Find Oligomers
          With Phenotypic Changes
          With Proposed Interfaces
          With In Vivo Evidence
          With Evidence for Physiological Relevance

If checked, GPCR-OKB will search for Protomers (i.e., constituent subunits of an Oligomer).

Please specify the name of any receptor protomer in the oligomer.

Example: If you would like to retrieve information about dopamine D2 receptor, you can either write "D2" or "dopamine".

Find Protomers

If checked, GPCR-OKB will search for Methods.

Please specify a method type used for the discovery and/or characterization of GPCR oligomers.

Example: If you would like to retrieve information about all oligomers identified by FRET studies, please write "FRET".

Find Methods

Boolean Operators
and	Specifies this term MUST be in the search results - this is the default operator
or	Specifies this term MAY be in the search results
not	Specifies this term MUST-NOT be in the search results

Search Term Modifiers
*	(Wildcard) Can be placed at the front and/or at the end of a search term to find partial term matches
~	(Regular Expression) Can be placed at the front of a search term to search using a regular expression

Special Notes
GPCR-OKB searches are always case-insensitive Any boolean operators provided should be preceding and connected to the term they operate on, for example use +cat not + cat (the extra space between + and cat will not work as desired) GPCR-OKB does not support phrasal or proximity searches

Examples
m1 or m2	Search for items containing the terms m1 or m2
m1 m2	Searches m1 and m2, as "and" is the default search operator
protein not m1 not m2	Search for items with the term protein but not the terms m1 or m2
rotei m2	Search for items which have a term that contains the string 'rotei' and the term m2
adenos* not *a	Search for items which have a term that starts with the string 'adenos' but no terms that end with the string 'a'

View a Study
The experimental or computational study used to study the oligomer to attempt to determine its existence, what its interacting domains are, and what its interacting residues are.

The type of method used.

Method Type

Method Type
Radioligand binding assay

The measurements made in an identification study using the given method type.

Measurements

Ligand Under Test

Measured Effect

Phenotypic Change

Ketanserin

Decreased

Type	Ligand	Compared With
LigandBinding	Ketanserin	mGLU2

2-[(1S,2S)-2-carboxycyclopropyl]-3-(9H-xanthen-9-yl)-D-alanine (LY-341495)

Increased

Type	Ligand	Compared With
LigandBinding	2-[(1S,2S)-2-carboxycyclopropyl]-3-(9H-xanthen-9-yl)-D-alanine (LY-341495)	mGLU2

The cell type in which the identification study was performed.

Cell Type

Human Brain

The article references from which the information in this object was derived.

Literature References

18297054 PubMed
Gonzalez-Maeso J, Ang RL, Yuen T, Chan P, Weisstaub NV, Lopez-Gimenez JF, Zhou M, Okawa Y, Callado LF, Milligan G, Gingrich JA, Filizola M, Meana JJ, and Sealfon SC. Identification of a serotonin/glutamate receptor complex implicated in psychosis. Nature 452 (2008 Mar 6): 93-7.

References:

Requirements and ontology for a G protein-coupled receptor oligomerization knowledge base. BMC Bioinformatics. 2007 May 30;8:177. Skrabanek L, Murcia M, Bouvier M, Devi L, George SR, Lohse MJ, Milligan G, Neubig R, Palczewski K, Parmentier M, Pin JP, Vriend G, Javitch JA, Campagne F, Filizola M.

GPCR-OKB: The G Protein Coupled Receptor Oligomer Knowledge Base. Bioinformatics. 2010 May 25. Khelashvili G, Dorff K, Shan J, Camacho-Artacho M, Skrabanek L, Vroling B, Bouvier M, Devi LA, George SR, Javitch JA, Lohse MJ, Milligan G, Neubig RR, Palczewski K, Parmentier M, Pin JP, Vriend G, Campagne F, Filizola M. doi:10.1093/bioinformatics/btq264

The G Protein Coupled Receptor -Oligomerization Knowledge Base Project

View a StudyThe experimental or computational study used to study the oligomer to attempt to determine its existence, what its interacting domains are, and what its interacting residues are.

The G Protein Coupled Receptor -
Oligomerization Knowledge Base Project

View a Study
The experimental or computational study used to study the oligomer to attempt to determine its existence, what its interacting domains are, and what its interacting residues are.